The airline chartered a Boeing 747 for a short flight circling the Netherlands, with 40 passengers on-board including senior politicians and business executives.
Dutch Economic Affairs Minister Maria van der Hoeven and KLM chief executive Peter Hartman were among those taking to the skies on the one-hour flight.
"This is technically feasible," Mr Hartman was quoted by the AFP news agency as saying after touching down safely in Schiphol Airport. "We have demonstrated that it is possible."
Issuing a call to action, he continued: "Government, industry and society at large must now join forces to ensure that we quickly gain access to a continuous supply of biofuel."
One of the Boeing 747's four mammoth engines was powered by the experimental mixture, which combines 50 per cent sustainable kerosene with 50 per cent normal fuel.
Many critics remain sceptical about the environmental benefits of biofuel – often citing concerns that they compete with indigenous food crops – but with the aviation sector pledging dramatic CO2 cuts in the decades ahead there appear to be few other viable alternatives.
Virgin Atlantic is also heavily involved in developing the technology, while Qatar Airways has taken a different tack by experimenting with natural gas as a jet fuel.
© Cheapflights Ltd (Creative Commons photo: bribriTO / Flickr)
Comments: